Matrix Science Pharma (MSP)

ABSTRACT

Objectives: The main objective of the study was to evaluate the antioxidant activities of Chaetomium cupreum extracts. Methods: The total flavonoid content was determined by using aluminum chloride method, whereas antioxidant activity (AA) was evaluated by ferric reducing antioxidant power assay, potassium ferricyanide reducing power assay, 2,2‑diphenyl‑1‑picyl‑hydrazyl method, β‑carotene bleaching assay, cupric ion reducing antioxidant capacity assay, lipid peroxidation inhibition assay by thiobarbituric acid (TBA)‑reactive substance method, and inhibition of hydrogen peroxide‑induced erythrocyte hemolysis assay Results: The ferric reducing AA of C. cupreum extracts at the concentration of 50 µg/mL was higher in ethyl acetate extract (6.11%) followed by chloroform extract (3.96%), n‑butanol extract (2.44%), and methanol extract (2.02%) mg RE/g dry weight. The potassium ferricyanide reducing activity of C. cupreum extracts at the concentration of 50 µg/mL was higher in ethyl acetate extract (15.90%) followed by chloroform (9.50%), n‑butanol (4.93%), and methanol extract (2.92%). The 2, 2‑diphenyl‑1‑picryl‑hydrazyl activity of C. cupreum extracts at 50 µg/mL was higher in ethyl acetate extract (36.13%) followed by n‑butanol extract (24.17%), chloroform extract (15.04%), and methanol extract (4.71%). The β‑carotene bleaching activity of C. cupreum extracts at 50 µg/mL after 1 h of incubation was higher in ethyl acetate extract at 12.88%, followed by chloroform extract (9.82%), n‑butanol extract (5.63%), and methanol extract (3.76%). The cupric ion reducing AA (CUPRAC) of C. cupreum extracts at 50 µg/mL was highest in the methanol extract (18.62%) followed by ethyl acetate extract (9.72%), n‑butanol extract (7.18%), and chloroform extract (2.46%) mg ACE/g dry weight. With regard to TBA reactive substance activity of C. cupreum extracts at 50 µg/mL, n‑butanol extract showed the highest lipid peroxidation inhibition (55.39%) followed by chloroform extract (50.51%), ethyl acetate extract (46.27%), and methanol extract (43.60%). With regard to the hydrogen peroxide‑induced hemolysis inhibition activity of C. cupreum extracts at the concentration of 500 µg/mL, ethyl acetate extract showed the highest inhibition (30.53%) followed by chloroform extract (26.42%) and n‑butanol extract (9.16%). Conclusions: The results of the present study showed that C. cupreum extracts poses significant antioxidant potential.