Matrix Science Pharma (MSP)

ABSTRACT

Objectives: To better understand the relationship between cell wall remodelling and fruit softening, an understanding of cell wall structure is necessary. Methods: Cellulose synthase gene that consists of cellulose synthase (CesA-like) plays important role in cellulose biosynthesis. However, CesA family genes are yet to be fully characterized in Solanaceae species. Results: In this study, we generated transgenic plants to test the role of CesA like gene in texture changes using tomato (Solanum lycopersicum) as a model system. We used the recently developed clustered regularly interspaced short palindromic repeat/Cas9 DNA editing technology to generate mutations in the target gene. Conclusions: Nevertheless, there was no mutation recovered in the CesA-like gene, and this indicates that this gene product is likely essential for regeneration of plantlets from tissue culture.