Objective: The evaluate acute and subacute toxicity of ethyl acetate extract of Oxalis latifolia and O. tetraphylla leaves (family: Oxalidaceae) in Swiss albino mice and Wistar albino rats. Methods: Swiss albino mice were used in the 500, 1000, and 2000 mg/kg acute toxicity investigations. Systematic measurements were taken at 1, 2, 3, and 24 hours post-dose. For the subacute toxicity, the animals were grouped into six groups of 6 rats where animals were fed with food and distilled water and the groups were control, 200 and 400 mg/kg of extracts respectively every 24 h orally for 28 days. Results: In acute toxicity studies, the extract did not induce death after dose up to 2000 mg/kg, the leaf ethyl acetate extracts from O. latifolia and O. tetraphylla did not result in any mortalities and were shown to be non-toxic to animals. After 28 days of treatment, there was no discernible difference in the body and organ weights between the treated group and the control group. Analysis of the extract's hemological composition and biochemical characteristics showed no harmful effects. Histopathological alterations were not present, nor were there any obvious abnormalities. In 28 days, no deaths were noted. Conclusions: The lack of toxicity at larger doses in rats suggests it may be worth exploring further for potential medical and therapeutic applications.